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Proc Natl Acad Sci U S A. 2016 Mar 15;113(11):3048-53. doi: 10.1073/pnas.1517719113. Epub 2016 Mar 14.

SARS-like WIV1-CoV poised for human emergence.

Author information

1
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
2
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
3
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079;
4
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
5
Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland; Humabs BioMed SA, Bellinzona, Switzerland;
6
Department of Cell Biology and Physiology and Marsico Lung Institute/Cystic Fibrosis Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
7
Institute for Research in Biomedicine, Bellinzona, Switzerland; Institute of Microbiology, Eidgenössische Technische Hochschule Zurich, Zurich, Switzerland;
8
Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute-Department of Medicine, Harvard Medical School, Boston MA 02215.
9
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079; rbaric@email.unc.edu.

Abstract

Outbreaks from zoonotic sources represent a threat to both human disease as well as the global economy. Despite a wealth of metagenomics studies, methods to leverage these datasets to identify future threats are underdeveloped. In this study, we describe an approach that combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emergence and pathogenic potential of circulating zoonotic viruses. Focusing on the severe acute respiratory syndrome (SARS)-like viruses, the results indicate that the WIV1-coronavirus (CoV) cluster has the ability to directly infect and may undergo limited transmission in human populations. However, in vivo attenuation suggests additional adaptation is required for epidemic disease. Importantly, available SARS monoclonal antibodies offered success in limiting viral infection absent from available vaccine approaches. Together, the data highlight the utility of a platform to identify and prioritize prepandemic strains harbored in animal reservoirs and document the threat posed by WIV1-CoV for emergence in human populations.

KEYWORDS:

CoV; SARS; Spike; WIV1; emergence

PMID:
26976607
PMCID:
PMC4801244
DOI:
10.1073/pnas.1517719113
[Indexed for MEDLINE]
Free PMC Article

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