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Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):E1863-71. doi: 10.1073/pnas.1600968113. Epub 2016 Mar 14.

A RhoA and Rnd3 cycle regulates actin reassembly during membrane blebbing.

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Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 819-0395, Japan;
Department of Advanced Information Technology, Kyushu University, Fukuoka 819-0395, Japan;
Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 819-0395, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan; AMED-PRIME, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan


The actin cytoskeleton usually lies beneath the plasma membrane. When the membrane-associated actin cytoskeleton is transiently disrupted or the intracellular pressure is increased, the plasma membrane detaches from the cortex and protrudes. Such protruded membrane regions are called blebs. However, the molecular mechanisms underlying membrane blebbing are poorly understood. This study revealed that epidermal growth factor receptor kinase substrate 8 (Eps8) and ezrin are important regulators of rapid actin reassembly for the initiation and retraction of protruded blebs. Live-cell imaging of membrane blebbing revealed that local reassembly of actin filaments occurred at Eps8- and activated ezrin-positive foci of membrane blebs. Furthermore, we found that a RhoA-ROCK-Rnd3 feedback loop determined the local reassembly sites of the actin cortex during membrane blebbing.


Eps8; Rnd3; actin cortex; cell migration; membrane bleb

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