Format

Send to

Choose Destination
Metabolism. 2016 Apr;65(4):522-32. doi: 10.1016/j.metabol.2015.12.011. Epub 2015 Dec 29.

Visualizing estrogen receptor-α-expressing neurons using a new ERα-ZsGreen reporter mouse line.

Author information

1
Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030.
2
Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030.
3
Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030; Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Electronic address: yongx@bcm.edu.

Abstract

BACKGROUND:

A variety of biological functions of estrogens, including regulation of energy metabolism, are mediated by neurons expressing estrogen receptor-α (ERα) in the brain. However, complex intracellular processes in these ERα-expressing neurons are difficult to unravel, due to the lack of strategy to visualize ERα-expressing neurons, especially in unfixed brain tissues.

RESULTS AND CONCLUSIONS:

Here we generated a novel ERα-ZsGreen reporter mouse line in which expression of a green fluorescent reporter protein, ZsGreen, is driven by a 241kb ERα gene promoter. We validated that ZsGreen is highly colocalized with endogenous ERα in the brain. Native ZsGreen signals were visualized in unfixed brain tissue, and were used to assist single cell collection and electrophysiological recordings. Finally, we demonstrated that this ERα-ZsGreen mouse allele can be used in combination with other genetic reporter alleles to allow experiments in highly selective neural populations.

KEYWORDS:

Brain; Electrophysiology; Estrogen receptor-α; Transgenic mouse

PMID:
26975544
PMCID:
PMC4794642
DOI:
10.1016/j.metabol.2015.12.011
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center