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Nat Cell Biol. 2016 Apr;18(4):451-7. doi: 10.1038/ncb3325. Epub 2016 Mar 14.

Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs.

Author information

1
Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, The Sahlgrenska Academy at the University of Gothenburg, Medicinaregatan 9A, 40539 Gothenburg, Sweden.
2
The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, Mill Hill, London NW7 1AA, UK.
3
Polarity, Division and Morphogenesis Team, Institut Curie, CNRS UMR 3215, INSERM U934, 26 rue d'Ulm, 75248 Paris Cedex 05, France.

Abstract

The apical and basolateral membranes of epithelia are insulated from each other, preventing the transfer of extracellular proteins from one side to the other. Thus, a signalling protein produced apically is not expected to reach basolateral receptors. Evidence suggests that Wingless, the main Drosophila Wnt, is secreted apically in the embryonic epidermis. However, in the wing imaginal disc epithelium, Wingless is mostly seen on the basolateral membrane where it spreads from secreting to receiving cells. Here we examine the apico-basal movement of Wingless in Wingless-producing cells of wing imaginal discs. We find that it is presented first on the apical surface before making its way to the basolateral surface, where it is released and allowed to interact with signalling receptors. We show that Wingless transcytosis involves dynamin-dependent endocytosis from the apical surface. Subsequent trafficking from early apical endosomes to the basolateral surface requires Godzilla, a member of the RNF family of membrane-anchored E3 ubiquitin ligases. Without such transport, Wingless signalling is strongly reduced in this tissue.

PMID:
26974662
PMCID:
PMC4817240
DOI:
10.1038/ncb3325
[Indexed for MEDLINE]
Free PMC Article

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