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Nat Cell Biol. 2016 Apr;18(4):382-92. doi: 10.1038/ncb3323. Epub 2016 Mar 14.

How the kinetochore couples microtubule force and centromere stretch to move chromosomes.

Author information

1
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
2
Department of Cell Biology, Duke University Medical Center, North Carolina 27710, USA.

Abstract

The Ndc80 complex (Ndc80, Nuf2, Spc24 and Spc25) is a highly conserved kinetochore protein essential for end-on anchorage to spindle microtubule plus ends and for force generation coupled to plus-end polymerization and depolymerization. Spc24/Spc25 at one end of the Ndc80 complex binds the kinetochore. The N-terminal tail and CH domains of Ndc80 bind microtubules, and an internal domain binds microtubule-associated proteins (MAPs) such as the Dam1 complex. To determine how the microtubule- and MAP-binding domains of Ndc80 contribute to force production at the kinetochore in budding yeast, we have inserted a FRET tension sensor into the Ndc80 protein about halfway between its microtubule-binding and internal loop domains. The data support a mechanical model of force generation at metaphase where the position of the kinetochore relative to the microtubule plus end reflects the relative strengths of microtubule depolymerization, centromere stretch and microtubule-binding interactions with the Ndc80 and Dam1 complexes.

PMID:
26974660
PMCID:
PMC4814359
DOI:
10.1038/ncb3323
[Indexed for MEDLINE]
Free PMC Article

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