Format

Send to

Choose Destination
PLoS One. 2016 Mar 14;11(3):e0151386. doi: 10.1371/journal.pone.0151386. eCollection 2016.

Transient Receptor Potential Melastatin-3 (TRPM3) Mediates Nociceptive-Like Responses in Hydra vulgaris.

Author information

1
Department of Science, University of Roma Tre, Rome, Italy.
2
Institute for Research on Pain, ISAL-Foundation, Torre Pedrera (RN), Italy.
3
IRCCS San Raffaele Pisana, Rome, Italy.
4
Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St Louis, United States of America.
5
IRC-FSH, Department of Health Science, University of 'Magna Graecia', Catanzaro, Italy.

Abstract

The ability of mammals to feel noxious stimuli lies in a heterogeneous group of primary somatosensory neurons termed nociceptors, which express specific membrane receptors, such as the Transient Receptor Potential (TRP) family. Here, we show that one of the most important nociceptive-like pathways is conserved in the freshwater coelenterate Hydra vulgaris, the most primitive organism possessing a nervous system. In particular, we found that H. vulgaris expresses TRPM3, a nociceptor calcium channel involved in the detection of noxious heat in mammals. Furthermore, we detected that both heat shock and TRPM3 specific agonist (i.e., pregnenolone sulfate) induce the modulation of the heat shock protein 70 (HSP70) and the nitric oxide synthase (NOS), two genes activated by TRP-mediated heat painful stimuli in mammals. As expected, these effects are inhibited by a TRPM3 antagonist (i.e., mefenamic acid). Interestingly, the TRPM3 agonist and heat shock also induce the expression of nuclear transcription erythroid 2-related factor (Nrf2) and superoxide dismutase (SOD), known markers of oxidative stress; noteworthy gene expression was also inhibited by the TRPM3 antagonist. As a whole, our results demonstrate the presence of conserved molecular oxidative/nociceptive-like pathways at the primordial level of the animal kingdom.

PMID:
26974325
PMCID:
PMC4790967
DOI:
10.1371/journal.pone.0151386
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center