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Sci Rep. 2016 Mar 14;6:23049. doi: 10.1038/srep23049.

Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis.

Author information

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
2
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Leninskie gory, 1/40, 119991 Moscow, Russia.
3
Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290 Moscow Region, Russia.

Abstract

Noggin4 is a Noggin family secreted protein whose molecular and physiological functions remain unknown. In this study, we demonstrate that in contrast to other Noggins, Xenopus laevis Noggin4 cannot antagonise BMP signalling; instead, it specifically binds to Wnt8 and inhibits the Wnt/β -catenin pathway. Live imaging demonstrated that Noggin4 diffusivity in embryonic tissues significantly exceeded that of other Noggins. Using the Fluorescence Recovery After Photobleaching (FRAP) assay and mathematical modelling, we directly estimated the affinity of Noggin4 for Wnt8 in living embryos and determined that Noggin4 fine-tune the Wnt8 posterior-to-anterior gradient. Our results suggest a role for Noggin4 as a unique, freely diffusing, long-range inhibitor of canonical Wnt signalling, thus explaining its ability to promote head development.

PMID:
26973133
PMCID:
PMC4789793
DOI:
10.1038/srep23049
[Indexed for MEDLINE]
Free PMC Article

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