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J Comp Neurol. 2016 Oct 1;524(14):2845-72. doi: 10.1002/cne.23995. Epub 2016 Apr 1.

Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations.

Author information

1
Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, Maryland, 21205-2185.
2
Department of Biological Structure, University of Washington and the Washington National Primate Research Center, Seattle, Washington, 98195-7420.
3
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston, Texas, 77030.
4
Department of Ophthalmology, John Hopkins University School of Medicine, Baltimore, Maryland, 21205-2185.
5
Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama, 35294.

Abstract

The long-term goal of this research is to understand how retinal ganglion cells that express the photopigment melanopsin, also known as OPN4, contribute to vision in humans and other primates. Here we report the results of anatomical studies using our polyclonal antibody specifically against human melanopsin that confirm and extend previous descriptions of melanopsin cells in primates. In macaque and human retina, two distinct populations of melanopsin cells were identified based on dendritic stratification in either the inner or the outer portion of the inner plexiform layer (IPL). Variation in dendritic field size and cell density with eccentricity was confirmed, and dendritic spines, a new feature of melanopsin cells, were described. The spines were the sites of input from DB6 diffuse bipolar cell axon terminals to the inner stratifying type of melanopsin cells. The outer stratifying melanopsin type received inputs from DB6 bipolar cells via a sparse outer axonal arbor. Outer stratifying melanopsin cells also received inputs from axon terminals of dopaminergic amacrine cells. On the outer stratifying melanopsin cells, ribbon synapses from bipolar cells and conventional synapses from amacrine cells were identified in electron microscopic immunolabeling experiments. Both inner and outer stratifying melanopsin cell types were retrogradely labeled following tracer injection in the lateral geniculate nucleus (LGN). In addition, a method for targeting melanopsin cells for intracellular injection using their intrinsic fluorescence was developed. This technique was used to demonstrate that melanopsin cells were tracer coupled to amacrine cells and would be applicable to electrophysiological experiments in the future. J. Comp. Neurol. 524:2845-2872, 2016.

KEYWORDS:

RRID:AB_397181; amacrine cell; bipolar cell; dopamine; intrinsically photosensitive ganglion cell; primate retina

PMID:
26972791
PMCID:
PMC4970949
DOI:
10.1002/cne.23995
[Indexed for MEDLINE]
Free PMC Article

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