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J Pharm Pharmacol. 2016 Apr;68(4):494-502. doi: 10.1111/jphp.12527. Epub 2016 Mar 9.

Tannic acid activates the Kv7.4 and Kv7.3/7.5 K(+) channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries.

Author information

1
Department of Pharmacology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China.
2
Department of Pharmacy, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
3
Department of Pharmacy, Maternal and Child Health Hospital, Tangshan, Hebei, China.
4
Vascular Surgery, The East Branch of Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
5
Intensive Care Unit, Air Force General Hospital, Beijing, Jiangsu, China.
6
Key Laboratory of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu, China.

Abstract

OBJECTIVES:

This study investigated the effect of tannic acid (TA), a plant-derived hydrolyzable polyphenol, on Kv7.4 and Kv7.5 K(+) channels and rat mesenteric artery.

METHODS:

Whole-cell patch clamp experiments were used to record the Kv7.4 and Kv7.3/7.5 K(+) currents expressed in HEK293 cells; and the tension changes of mesenteric arteries isolated from rats were recorded using small vessel myography apparatus.

KEY FINDINGS:

Tannic acid increases the Kv7.4 and Kv7.3/7.5 K(+) currents in a concentration-dependent manner (median effective concentration (EC50 ) = 27.3 ± 3.6 μm and EC50 = 23.1 ± 3.9 μm, respectively). In addition, 30 μm TA shifts the G-V curve of Kv7.4 and Kv7.3/7.5 K(+) currents to the left by 14.18 and 25.24 mV, respectively, and prolongs the deactivation time constants by 184.44 and 154.77 ms, respectively. Moreover, TA relaxes the vascular tension of rat mesenteric arteries in a concentration-dependent manner (half inhibitory concentration (IC50 ) = 148.7 ± 13.4 μm).

CONCLUSION:

These results confirms the vasodilatory effects of TA on rat mesenteric artery and the activating effects on the Kv7.4 and Kv7.3/7.5 K(+) channels, which may be a mechanism to explain the vasodilatory effect and this mechanism can be used in the research of antihypertension.

KEYWORDS:

K+ channels; antihypertension; tannic acid; vascular tension

PMID:
26969140
DOI:
10.1111/jphp.12527
[Indexed for MEDLINE]

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