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J Vet Intern Med. 2016 May;30(3):764-70. doi: 10.1111/jvim.13924. Epub 2016 Mar 10.

Comparison of Serum Spec fPL(™) and 1,2-o-Dilauryl-Rac-Glycero-3-Glutaric Acid-(6'-Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology.

Author information

1
Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
2
Institute for Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
3
Section of Epidemiology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
4
Department of Animal Medicine, Production and Health, University of Padova, Legnaro, PD, Italy.
5
Istituto Veterinario di Novara, Granozzo con Monticello, NO, Italy.

Abstract

BACKGROUND:

Feline pancreas-specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking.

OBJECTIVES:

To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas.

ANIMALS:

Sixty client-owned cats presented for necropsy.

METHODS:

PROSPECTIVE STUDY:

Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined.

RESULTS:

The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4-55.9%] and 100% (95% CI, 31.0-100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7-50.7%) and 100% (95% CI, 31.0-100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1-81.7%) and 69.0% (95% CI, 52.8-81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2-85.6%) and 78.6% (95% CI, 62.8-89.2%).

CONCLUSIONS AND CLINICAL IMPORTANCE:

Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited.

KEYWORDS:

Feline; Feline pancreas-specific lipase; Histopathology; Lipase; Pancreas

PMID:
26968865
PMCID:
PMC4913568
DOI:
10.1111/jvim.13924
[Indexed for MEDLINE]
Free PMC Article

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