Format

Send to

Choose Destination
Cancer Immunol Res. 2016 May;4(5):412-418. doi: 10.1158/2326-6066.CIR-15-0240. Epub 2016 Mar 11.

TCR Sequencing Can Identify and Track Glioma-Infiltrating T Cells after DC Vaccination.

Author information

1
Department of Neurosurgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
2
Department of Pediatrics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
3
Department of Medicine (Hematology/Oncology), David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
4
The Medical Scientist Training Program, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
5
Department of Biostatistics, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
6
Adaptive Biotechnologies, Seattle, WA, USA.
7
Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
8
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
9
Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
10
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
#
Contributed equally

Abstract

Although immunotherapeutic strategies are emerging as adjunctive treatments for cancer, sensitive methods of monitoring the immune response after treatment remain to be established. We used a novel next-generation sequencing approach to determine whether quantitative assessments of tumor-infiltrating lymphocyte (TIL) content and the degree of overlap of T-cell receptor (TCR) sequences in brain tumors and peripheral blood were predictors of immune response and overall survival in glioblastoma patients treated with autologous tumor lysate-pulsed dendritic cell immunotherapy. A statistically significant correlation was found between a higher estimated TIL content and increased time to progression and overall survival. In addition, we were able to assess the proportion of shared TCR sequences between tumor and peripheral blood at time points before and after therapy, and found the level of TCR overlap to correlate with survival outcomes. Higher degrees of overlap, or the development of an increased overlap following immunotherapy, was correlated with improved clinical outcome, and may provide insights into the successful, antigen-specific immune response. Cancer Immunol Res; 4(5); 412-8.

PMID:
26968205
PMCID:
PMC4873445
DOI:
10.1158/2326-6066.CIR-15-0240
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center