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J Vis Exp. 2016 Mar 4;(109):e53075. doi: 10.3791/53075.

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome.

Author information

1
Academic Geriatric Medicine, University of Southampton, University Hospital Southampton; National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust.
2
MRC Lifecourse Epidemiology Unit, University of Southampton.
3
Academic Geriatric Medicine, University of Southampton, University Hospital Southampton; National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust; MRC Lifecourse Epidemiology Unit, University of Southampton; National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care.
4
National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust; MRC Lifecourse Epidemiology Unit, University of Southampton; National Institute for Health Research Musculoskeletal Biomedical Research Unit, University of Oxford.
5
Academic Geriatric Medicine, University of Southampton, University Hospital Southampton; National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust; MRC Lifecourse Epidemiology Unit, University of Southampton; National Institute for Health Research Collaboration for Leadership in Applied Health Research and Care; Newcastle University Institute of Ageing and Institute of Health and Society, Newcastle University.
6
Academic Geriatric Medicine, University of Southampton, University Hospital Southampton; National Institute for Health Research Southampton Biomedical Research Center, University of Southampton and University Hospital Southampton NHS Foundation Trust; MRC Lifecourse Epidemiology Unit, University of Southampton; hp@mrc.soton.ac.uk.

Abstract

Percutaneous muscle biopsy using the Weil-Blakesley conchotome is well established in both clinical and research practice. It is a safe, effective and well tolerated technique. The Weil-Blakesley conchotome has a sharp biting tip with a 4 - 6 mm wide hollow. It is inserted through a 5 - 10 mm skin incision and can be maneuvered for controlled tissue penetration. The tip is opened and closed within the tissue and then rotated through 90 -180° to cut the muscle. The amount of muscle obtained following repeated sampling can vary from 20 mg to 290 mg which can be processed for both histology and molecular studies. The wound needs to be kept dry and vigorous physical activity kept to a minimum for approximately 72 hr although normal levels of activity can restart immediately following the procedure. This procedure is safe and effective when close attention is paid to the selection of subjects, full asepsis and post procedure care.  Both right and left vastus lateralis are suitable for biopsy dependent on participant preference.

PMID:
26967381
PMCID:
PMC4828222
DOI:
10.3791/53075
[Indexed for MEDLINE]
Free PMC Article

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