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Environ Sci Pollut Res Int. 2017 May;24(14):12740-12747. doi: 10.1007/s11356-016-6316-y. Epub 2016 Mar 11.

Subtype analysis of Giardia duodenalis isolates from municipal and domestic raw wastewaters in Iran.

Author information

1
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
2
Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
3
Department of Environmental Health Engineering, School of Public Health, and Center for Water Quality Research, Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran.
4
Gastroenterology and Liver Disease Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5
Foodborne and Waterborne Diseases Research Center, Research institute for Gastroentrology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6
Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
7
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. rezaiian@tums.ac.ir.

Abstract

A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses in Tehran, Iran, were assessed for the presence of the Giardia cysts using immunofluorescence with monoclonal antibodies. To characterize the cysts at the molecular level, the three genetic loci were amplified and sequenced. The assemblages A (37.5 %) and E (58.3 %) were detected in livestock wastewater samples. Assemblage A, which is composed of only G. duodenalis genotype, was detected in 100 % of urban wastewater samples. The subassemblages A2, A3, A-I, A-II, and E3 were identified with β-giardin, triose phosphate isomerase, and glutamate dehydrogenase genes. This study is the first to report on G. duodenalis genotypes in aquatic environmental samples in Iran.

KEYWORDS:

Giardia duodenalis; Glutamate dehydrogenase (GDH); Iran; Triose phosphate isomerase (TPI); Wastewater; β-giardin gene (BG)

PMID:
26965275
DOI:
10.1007/s11356-016-6316-y
[Indexed for MEDLINE]

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