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Biofouling. 2016;32(4):489-96. doi: 10.1080/08927014.2016.1148693.

Epithelial cell detachment by Porphyromonas gingivalis biofilm and planktonic cultures.

Author information

1
a Department of Operative Dentistry and Endodontics , Guanghua School of Stomatology, Sun Yat-sen University , Guangzhou , PR China.
2
b Department of Preventive Dentistry , Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam , Amsterdam , The Netherlands.
3
c Guangdong Provincial Key Laboratory of Stomatology , Sun Yat-sen University , Guangzhou , PR China.

Abstract

Porphyromonas gingivalis is present as a biofilm at the sites of periodontal infections. The detachment of gingival epithelial cells induced by P. gingivalis biofilms was examined using planktonic cultures as a comparison. Exponentially grown planktonic cultures or 40-h biofilms were co-incubated with epithelial cells in a 24-well plate for 4 h. Epithelial cell detachment was assessed using imaging. The activity of arginine-gingipain (Rgp) and gene expression profiles of P. gingivalis cultures were examined using a gingipain assay and quantitative PCR, respectively. P. gingivalis biofilms induced significantly higher cell detachment and displayed higher Rgp activity compared to the planktonic cultures. The genes involved in gingipain post-translational modification, but not rgp genes, were significantly up-regulated in P. gingivalis biofilms. The results underline the importance of including biofilms in the study of bacterial and host cell interactions.

KEYWORDS:

Cytotoxicity; detachment; gingipain; gingival epithelial cells; glycosyltransferase; quantitative PCR

PMID:
26963862
DOI:
10.1080/08927014.2016.1148693
[Indexed for MEDLINE]

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