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ACS Chem Biol. 2016 May 20;11(5):1438-44. doi: 10.1021/acschembio.5b01054. Epub 2016 Mar 16.

Reaction of PerR with Molecular Oxygen May Assist H2O2 Sensing in Anaerobes.

Sethu R1,2,3, Gouré E1,2,3, Signor L4,5,6, Caux-Thang C1,2,3, Clémancey M1,2,3, Duarte V1,2,3, Latour JM1,2,3.

Author information

1
Université Grenoble Alpes, LCBM , F-38054 Grenoble, France.
2
CEA, DSV, BIG, LCBM, PMB , F-38054 Grenoble, France.
3
CNRS UMR 5249, LCBM , F-38054 Grenoble, France.
4
Université Grenoble Alpes, IBS , F-38044 Grenoble, France.
5
CNRS, IBS , F-38044 Grenoble, France.
6
CEA, IBS , F-38044 Grenoble, France.

Abstract

PerR is the peroxide resistance regulator found in several pathogenic bacteria and governs their resistance to peroxide stress by inducing enzymes that destroy peroxides. However, it has recently been implicated as a key component of the aerotolerance in several facultative or strict anaerobes, including the highly pathogenic Staphylococcus aureus. By combining (18)O labeling studies to ESI- and MALDI-TOF MS detection and EMSA experiments, we demonstrate that the active form of PerR reacts with dioxygen, which leads ultimately to disruption of the PerR/DNA complex and is thus physiologically meaningful. Moreover, we show that the presence of O2 assists PerR sensing of H2O2, another feature likely to be important for anaerobic organisms. These results allow one to envisage different scenarios for the response of anaerobes to air exposure.

PMID:
26963368
DOI:
10.1021/acschembio.5b01054
[Indexed for MEDLINE]

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