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Mol Med Rep. 2016 Apr;13(4):3459-65. doi: 10.3892/mmr.2016.4978. Epub 2016 Mar 4.

Impaired function of the intestinal barrier in a novel sub-health rat model.

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Department of Clinical Laboratory, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
Department of Clinical Laboratory, Hunan Guangxiu Hospital, Changsha, Hunan 410000, P.R. China.
Hepatobiliary and Enteric Surgery Research Center, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.


Sub-health is a state featuring a deterioration in physiological function between health and illness, and the sub-health condition has surfaced as life-threatening in humans. The aim of the present study was to establish a sub-health model in rats, and investigate the function of the intestinal barrier in the sub-health rats and rats following intervention. To establish a sub‑health model, the rats were subjected to a high‑fat and sugar diet, motion restriction and chronic stress. Their serum glucose and triglyceride levels, immune function and adaptability were then measured. The levels of diamine oxidase and D‑lactic acid in the plasma were analyzed as markers of the intestinal permeability. The protein and mRNA expression levels of anti‑apoptotic YWHAZ in the colonic tissue was detected using immunohistochemical and reverse transcription‑quantitative polymerase chain reaction analyses In the present study, the sub‑health rat model was successfully established, and sub‑health factors increased the intestinal permeability and reduced the expression of YWHAZ. Providing sub‑health rats with normal living conditions did not improve the function of the intestinal barrier. In conclusion, the results of the present study demonstrated that intestinal disorders in the sub‑health rat model may result from the damage caused by reduce intestinal barrier function as well as the decreased expression levels of YWHAZ. Additionally, rats in the sub‑health condition did not recover following subsequent exposure to normal living conditions, suggesting that certain exercises or medical intervention may be necessary to improve sub-health symptoms.

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