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Stem Cells Dev. 2016 May 1;25(9):729-39. doi: 10.1089/scd.2015.0386. Epub 2016 Apr 11.

CD30 Receptor-Targeted Lentiviral Vectors for Human Induced Pluripotent Stem Cell-Specific Gene Modification.

Author information

1
1 Molecular Biotechnology and Gene Therapy, Paul-Ehrlich-Institut , Langen, Germany .
2
2 Medical Biotechnology, Paul-Ehrlich-Institut , Langen, Germany .
3
3 Research Group for Gene Modification in Stem Cells, LOEWE Center of Cell and Gene Therapy Frankfurt , Paul-Ehrlich-Institut, Langen, Germany .

Abstract

Cultures of induced pluripotent stem cells (iPSCs) often contain cells of varying grades of pluripotency. We present novel lentiviral vectors targeted to the surface receptor CD30 (CD30-LV) to transfer genes into iPSCs that are truly pluripotent as demonstrated by marker gene expression. We demonstrate that CD30 expression is restricted to SSEA4(high) cells of human iPSC cultures and a human embryonic stem cell line. When CD30-LV was added to iPSCs during routine cultivation, efficient and exclusive transduction of cells positive for the pluripotency marker Oct-4 was achieved, while retaining their pluripotency. When added during the reprogramming process, CD30-LV solely transduced cells that became fully reprogrammed iPSCs as confirmed by co-expression of endogenous Nanog and the reporter gene. Thus, CD30-LV may serve as novel tool for the selective gene transfer into PSCs with broad applications in basic and therapeutic research.

PMID:
26956718
DOI:
10.1089/scd.2015.0386
[Indexed for MEDLINE]

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