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Spectrochim Acta A Mol Biomol Spectrosc. 2016 May 15;161:77-82. doi: 10.1016/j.saa.2016.02.036. Epub 2016 Mar 3.

Protein-binding, cytotoxicity in vitro and cell cycle arrest of ruthenium(II) polypyridyl complexes.

Author information

1
Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
2
Department of Pharmacy, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
3
Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China. Electronic address: wuyong@gzhmu.edu.cn.
4
Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China. Electronic address: qfguo@gzhmu.edu.cn.

Abstract

The cytotoxic activity of two Ru(II) complexes against A549, BEL-7402, HeLa, PC-12, SGC-7901 and SiHa cell lines was investigated by MTT method. Complexes 1 and 2 show moderate cytotoxicity toward BEL-7402 cells with an IC50 value of 53.9 ± 3.4 and 39.3 ± 2.1 μM. The effects of the complexes inducing apoptosis, cellular uptake, reactive oxygen species and mitochondrial membrane potential in BEL-7402 cells have been studied by fluorescence microscopy. The percentages of apoptotic and necrotic cells and cell cycle arrest were studied by flow cytometry. The BSA-binding behaviors were investigated by UV/visible and fluorescent spectra.

KEYWORDS:

Apoptosis; BSA-binding; Cell cycle arrest; Cytotoxicity in vitro; Ru(II) complexes

PMID:
26956530
DOI:
10.1016/j.saa.2016.02.036
[Indexed for MEDLINE]

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