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Microb Drug Resist. 2016 Mar;22(2):129-33. doi: 10.1089/mdr.2015.0157. Epub 2015 Aug 20.

Outbreak Caused by blaOXA-72-Producing Acinetobacter baumannii ST417 Detected in Clinical and Environmental Isolates.

Author information

1
1 Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública , Cuernavaca, México.
2
2 Laboratorio de Genética y Diagnóstico Molecular and Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí , San Luis Potosí, México.
3
3 Laboratorio de Microbiología, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí , México.
4
4 Hospital Central Dr. Ignacio Morones Prieto , San Luis Potosí, México.
5
5 Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México , Cuernavaca, México.

Abstract

We characterized an outbreak of imipenem-resistant Acinetobacter baumannii with clinical and environmental isolates from a tertiary care hospital in San Luis Potosi, Mexico. During a 4-month period, a total of 32 nonrepetitive imipenem-resistant clinical isolates of A. baumannii were collected. All isolates were susceptible to colistin and tigecycline and resistant to cefepime, ceftazidime, ceftriaxone, imipenem, and meropenem. Genotyping by pulsed-field gel electrophoresis showed a major clone (A). Multilocus sequence type (MLST) analysis was performed, revealing sequence type (ST) 417 (ST417) and 208 (ST208). The blaIMP-, blaVIM-, blaGIM-, blaSIM-, blaNDM-type, and blaOXA-type (blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like) genes were screened and showed that the blaOXA-51-like and blaOXA-24-like genes were present in all isolates. Sequencing and southern hybridization were performed, confirming the presence of the blaOXA-72 gene and its plasmid-borne nature. In addition, the blaOXA-72-XerC/XerD-like association was identified. These findings indicate that a clonal spread of blaOXA-72-producing A. baumannii ST417 had occurred throughout the hospital. The ST417 corresponded with a previous ST described in the United States.

PMID:
26954369
DOI:
10.1089/mdr.2015.0157
[Indexed for MEDLINE]

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