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Bioresour Technol. 2016 Jun;209:80-9. doi: 10.1016/j.biortech.2016.02.098. Epub 2016 Feb 27.

Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis.

Author information

1
Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Sendai 9808579, Japan; Department of Agricultural Microbiology, Agriculture and Biology Research Division, National Research Centre, Dokki, Cairo 12622, Egypt.
2
Department of Environmental Science, Graduate School of Environmental Studies, Tohoku University, Sendai 9808579, Japan.
3
Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Sendai 9808579, Japan; Department of Environmental Science, Graduate School of Environmental Studies, Tohoku University, Sendai 9808579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

Abstract

A series of standardized activity experiments were performed to characterize three different temperature ranges of hydrogen fermentation from different carbon sources. 16S rRNA sequences analysis showed that the bacteria were close to Enterobacter genus in the mesophilic mixed culture (MMC) and Thermoanaerobacterium genus in the thermophilic and hyper-thermophilic mixed cultures (TMC and HMC). The MMC was able to utilize the glucose and cellulose to produce methane gas within a temperature range between 25 and 45 °C and hydrogen gas from 35 to 60°C. While, the TMC and HMC produced only hydrogen gas at all temperature ranges and the highest activity of 521.4mlH2/gVSSd was obtained by TMC. The thermodynamic analysis showed that more energy is consumed by hydrogen production from cellulose than from glucose. The experimental results could help to improve the economic feasibility of cellulosic biomass energy using three-phase technology to produce hythane.

KEYWORDS:

16s rRNA; Bio-hydrogen; Cellulose; Mixed culture; Temperature variation

PMID:
26954308
DOI:
10.1016/j.biortech.2016.02.098
[Indexed for MEDLINE]

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