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Gene. 1989 Dec 21;85(1):253-8.

Characterization of a cryptic plasmid from Lactobacillus plantarum.

Author information

1
Department of Agricultural Biochemistry and Nutrition, University of Newcastle upon Tyne, U.K.

Abstract

The complete nucleotide sequence of pLB4, a cryptic plasmid isolated from Lactobacillus plantarum NCDO1088 has been determined. Three open reading frames, which encode proteins of 42, 25 and 6 kDa, have been identified. In vitro transcription/translation of pLB4-derived DNA restriction fragments confirm the existence of all three polypeptides, which show homology to replication proteins and site-specific recombinases from other Gram+ plasmids. Three major regions of dyad symmetry with delta G of -28.8, -15.0 and -17.0 kcal were observed. One of these regions contains a sequence which shows perfect homology to the nick site of the Gram+ replicons, pE194, pLS1 and pADB201. In addition, a 21-bp sequence located upstream from the site-specific recombinase shows 80% homology to the recombination sites of pE194 and pT181.

PMID:
2695401
DOI:
10.1016/0378-1119(89)90491-5
[Indexed for MEDLINE]

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