Format

Send to

Choose Destination
Genetics. 2016 Mar;202(3):885-901. doi: 10.1534/genetics.115.182162.

CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering.

Author information

1
Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280 ddickins@live.unc.edu.
2
Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599-3280.

Abstract

The advent of genome editing techniques based on the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system has revolutionized research in the biological sciences. CRISPR is quickly becoming an indispensible experimental tool for researchers using genetic model organisms, including the nematode Caenorhabditis elegans. Here, we provide an overview of CRISPR-based strategies for genome editing in C. elegans. We focus on practical considerations for successful genome editing, including a discussion of which strategies are best suited to producing different kinds of targeted genome modifications.

KEYWORDS:

CRISPR/Cas9; Caenorhabditis elegans; WormBook; genome editing

PMID:
26953268
PMCID:
PMC4788126
DOI:
10.1534/genetics.115.182162
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center