Format

Send to

Choose Destination
EMBO Rep. 2016 May;17(5):695-707. doi: 10.15252/embr.201541060. Epub 2016 Mar 7.

Sororin loads to the synaptonemal complex central region independently of meiotic cohesin complexes.

Author information

1
Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain rocio.gomez@uam.es jose.suja@uam.es.
2
Instituto de Biología Molecular y Celular del Cáncer (CSIC-Universidad de Salamanca) Campus Miguel de Unamuno, Salamanca, Spain.
3
Chromosome Dynamics Group, Centro Nacional de Investigaciones Oncológicas CNIO, Madrid, Spain.
4
Unidad de Biología Celular, Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
5
Departamento de Biología Celular y Molecular, Centro de Investigaciones Biológicas CSIC, Madrid, Spain.
6
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan.
7
Department of Cell and Developmental Biology, Biocenter University of Würzburg, Würzburg, Germany.

Abstract

The distribution and regulation of the cohesin complexes have been extensively studied during mitosis. However, the dynamics of their different regulators in vertebrate meiosis is largely unknown. In this work, we have analyzed the distribution of the regulatory factor Sororin during male mouse meiosis. Sororin is detected at the central region of the synaptonemal complex during prophase I, in contrast with the previously reported localization of other cohesin components in the lateral elements. This localization of Sororin depends on the transverse filaments protein SYCP1, but not on meiosis-specific cohesin subunits REC8 and SMC1β. By late prophase I, Sororin accumulates at centromeres and remains there up to anaphase II The phosphatase activity of PP2A seems to be required for this accumulation. We hypothesize that Sororin function at the central region of the synaptonemal complex could be independent on meiotic cohesin complexes. In addition, we suggest that Sororin participates in the regulation of centromeric cohesion during meiosis in collaboration with SGO2-PP2A.

KEYWORDS:

Sororin; centromere; cohesin complex; meiosis; synaptonemal complex

PMID:
26951638
PMCID:
PMC5341523
DOI:
10.15252/embr.201541060
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center