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Nat Neurosci. 2016 Apr;19(4):554-6. doi: 10.1038/nn.4266. Epub 2016 Mar 7.

Biophysical constraints of optogenetic inhibition at presynaptic terminals.

Author information

1
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.

Abstract

We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Thus, the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.

PMID:
26950004
PMCID:
PMC4926958
DOI:
10.1038/nn.4266
[Indexed for MEDLINE]
Free PMC Article

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