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Sci Rep. 2016 Mar 7;6:22919. doi: 10.1038/srep22919.

Whole genome sequencing identifies a novel species of the genus Capnocytophaga isolated from dog and cat bite wounds in humans.

Author information

1
Department of Laboratory Medicine, Div of Clinical Microbiology, Karolinska Institutet and Karolinska University Hospital, Huddinge, Sweden.
2
KTH Royal Institute of Technology, Science for Life Laboratory, School of Biotechnology, Division of Gene Technology, Stockholm, Sweden.
3
Evolutionary Genomics Group, Departamento de Producción Vegetal y Microbiología,Universidad Miguel Hernández, San Juan de Alicante, 03550, Alicante, Spain.

Abstract

C. canimorsus and C. cynodegmi are dog and cat commensals which can be transmitted to humans via bites or scratches and can cause sepsis, meningitis, endocarditis, and eye- or wound infections. Recently an additional Capnocytophaga species was identified as part of the oral flora of healthy dogs and was given the name "C. canis". We previously identified a Capnocytophaga isolate that could not be typed with available diagnostic tests including MALDI-TOF, 16S rRNA sequencing or species-specific PCR. This strain and 21 other Capnocytophaga spp isolated in Sweden from clinical blood- or wound-cultures were subjected to whole genome sequencing using the Illumina platform. Phylogenetic analysis revealed that the previously non-typable isolate belongs to the putative new species "C. canis". Since this strain was isolated from a wound it also shows that members of "C. canis" have the potential to be pathogenic. In addition, our phylogenetic analysis uncovered an additional species of Capnocytophaga, which can be transmitted from dogs and cats to humans, suggesting a speciation within the Capnocytophaga family that has not been observed before. We propose the name of "C. stomatis" for this putative novel species.

PMID:
26947740
PMCID:
PMC4780008
DOI:
10.1038/srep22919
[Indexed for MEDLINE]
Free PMC Article

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