Format

Send to

Choose Destination
J Infect. 2016 Jun;72(6):723-730. doi: 10.1016/j.jinf.2016.02.016. Epub 2016 Mar 4.

Conventional and innate lymphocytes response at the acute phase of HEV infection in transplanted patients.

Author information

1
Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, Centre National de Référence Hépatite E, Institut fédératif de biologie de Purpan, F-31300, Toulouse, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France. Electronic address: abravanel.f@chu-toulouse.fr.
2
Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France.
3
Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Rangueil, Service de Néphrologie, Dialyse et Transplantation multi-organe, F-31049, Toulouse, France.
4
Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, Centre National de Référence Hépatite E, Institut fédératif de biologie de Purpan, F-31300, Toulouse, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France.
5
CHU Toulouse, Hôpital Purpan, Département de Gastroentérologie, F-31300, Toulouse, France.
6
CHU Toulouse, Hôpital Purpan, Service de médecine interne, F-31300, Toulouse, France.
7
Inserm, U1043, Toulouse, F-31300, France; CNRS, U5282, Toulouse, F-31300, France; Université Toulouse, UPS, Centre de Physiopathologie de Toulouse (CPTP), F-31300, Toulouse, France; CHU Toulouse, Hôpital Rangueil, Service de Néphrologie, Dialyse et Transplantation multi-organe, F-31049, Toulouse, France.

Abstract

OBJECTIVES:

The hepatitis E virus (HEV) causes usually benign and spontaneously resolving acute hepatitis in immunocompetent individuals. In immunocompromised patients with a solid-organ transplant (SOT), chronic infections occur in about 2/3 of cases. We aimed to evaluate the immune cells implicated at the acute phase of HEV infection.

METHODS:

We studied the activation and memory markers on CD4, CD8, γδ and NK cells in 32 HEV-free control SOT patients and 23 SOT recipients, including 14 who became chronically infected. Samples from 7 immunocompetent individuals with an acute infection and 8 healthy donor samples were included for comparison.

RESULTS:

In acutely-infected SOT patients, NK and Vδ2 cells, but not other γδ cells, had an increased expression of CD69. Based on CD45RA/CD27 markers, solid-organ recipients infected with HEV contained a larger pool of circulating naive subsets among lymphocyte Tγδ cells. However, these alterations of Vδ2 cells were not associated with HEV clearance. Only the adaptive IFN-γ responses to HEV peptides, determined by ELISpot, were associated with a favorable outcome in immunocompromised patients.

CONCLUSIONS:

Transplanted patients mobilized their γδ cells at the acute phase of infection. Their precise role in HEV infection will thus deserve further investigations as they could be specifically immunomanipulated.

KEYWORDS:

Chronic viral hepatitis; Gamma-delta T cells; Hepatitis E virus; Solid organ transplantation

PMID:
26947133
DOI:
10.1016/j.jinf.2016.02.016
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center