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Nat Commun. 2016 Mar 7;7:10866. doi: 10.1038/ncomms10866.

CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits.

Böhm UL1,2,3,4, Prendergast A1,2,3,4, Djenoune L1,2,3,4,5, Nunes Figueiredo S1,2,3,4, Gomez J1,2,3,4, Stokes C1,2,3,4, Kaiser S1,2,3,4, Suster M6,7, Kawakami K7, Charpentier M5, Concordet JP5, Rio JP1,2,3,4, Del Bene F8,9,10, Wyart C1,2,3,4.

Author information

1
Institut du Cerveau et de la Moelle épinière, Paris 75013, France.
2
UPMC Univ. Paris 06, Paris 75005, France.
3
Inserm UMR 1127, Paris 75013, France.
4
CNRS UMR 7225, Paris 75013, France.
5
Muséum National d'Histoire Naturelle, Paris 75005, France.
6
Neural Circuits and Behaviour Group, University of Bergen, Bergen 5008, Norway.
7
Division of Molecular and Developmental Biology, National Institute of Genetics and Department of Genetics, Sokendai (The Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, Japan.
8
Institut Curie, Paris 75005, France.
9
CNRS UMR 3215, Paris 75005, France.
10
Inserm U 934, Paris 75005, France.

Abstract

Throughout vertebrates, cerebrospinal fluid-contacting neurons (CSF-cNs) are ciliated cells surrounding the central canal in the ventral spinal cord. Their contribution to modulate locomotion remains undetermined. Recently, we have shown CSF-cNs modulate locomotion by directly projecting onto the locomotor central pattern generators (CPGs), but the sensory modality these cells convey to spinal circuits and their relevance to innate locomotion remain elusive. Here, we demonstrate in vivo that CSF-cNs form an intraspinal mechanosensory organ that detects spinal bending. By performing calcium imaging in moving animals, we show that CSF-cNs respond to both passive and active bending of the spinal cord. In mutants for the channel Pkd2l1, CSF-cNs lose their response to bending and animals show a selective reduction of tail beat frequency, confirming the central role of this feedback loop for optimizing locomotion. Altogether, our study reveals that CSF-cNs constitute a mechanosensory organ operating during locomotion to modulate spinal CPGs.

PMID:
26946992
PMCID:
PMC4786674
DOI:
10.1038/ncomms10866
[Indexed for MEDLINE]
Free PMC Article

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