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J Neuropathol Exp Neurol. 2016 Apr;75(4):295-8. doi: 10.1093/jnen/nlw010. Epub 2016 Mar 4.

SOX10 Distinguishes Pilocytic and Pilomyxoid Astrocytomas From Ependymomas but Shows No Differences in Expression Level in Ependymomas From Infants Versus Older Children or Among Molecular Subgroups.

Author information

1
From the Department of Pathology (BKKD, AMR); Department of Neurology (BKKD); and Department of Neurosurgery, University of Colorado Health Sciences Center (BKKD), Aurora, Colorado; Department of Neuro-Oncology, Children's Hospital Colorado (AMD, NKF), Aurora, Colorado; and Department of Pathology, University of California San Francisco (MP, TT), San Francisco, California. bk.demasters@ucdenver.edu.
2
From the Department of Pathology (BKKD, AMR); Department of Neurology (BKKD); and Department of Neurosurgery, University of Colorado Health Sciences Center (BKKD), Aurora, Colorado; Department of Neuro-Oncology, Children's Hospital Colorado (AMD, NKF), Aurora, Colorado; and Department of Pathology, University of California San Francisco (MP, TT), San Francisco, California.

Abstract

SOX10 is important in nonneoplastic oligodendroglial development, but mRNA transcripts and protein expression are identified in a wider variety of CNS glial neoplasms than oligodendrogliomas. We previously demonstrated high levels of SOX10 mRNA and protein in pilocytic astrocytomas (PAs) but not ependymomas (EPNs). We now extend these studies to investigate subsets of these 2 tumors that affect infants, pilomyxoid astrocytomas (PMAs) and infant (<1 year) ependymomas (iEPNs). By gene expression microarray analysis, we found that iEPNs and all EPNs in older children showed very low SOX10 expression levels, on average 7.1-fold below normal control tissues. EPN groups showed no significant difference in SOX10 expression between iEPN and EPN. PAs/PMAs had 24.1/29.4-fold higher transcript levels, respectively, than those in normal tissues. Using immunohistochemical analysis of adult, pediatric, and infantile EPNs and of PAs/PMAs, we found that EPNs from multiple anatomical locations and both age groups (n = 228) never showed 3+ diffuse nuclear immunostaining for SOX10; the majority were scored at 0 or 1+. Conversely, almost all pediatric and adult PAs and PMAs (n = 47) were scored as 3+. These results suggest that in select settings, SOX10 immunohistochemistry can supplement the diagnosis of PMA and PA and aid in distinguishing them from EPNs.

KEYWORDS:

Developmental; Gene expression microarray; Infant ependymoma; Myxopapillary ependymoma; Pilocytic astrocytoma; Pilomyxoid astrocytoma; SOX10.

PMID:
26945037
PMCID:
PMC5009481
DOI:
10.1093/jnen/nlw010
[Indexed for MEDLINE]
Free PMC Article

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