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Trends Biotechnol. 2016 Jul;34(7):575-587. doi: 10.1016/j.tibtech.2016.02.004. Epub 2016 Mar 2.

Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit.

Author information

1
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
2
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands; Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
3
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands. Electronic address: john.vanderoost@wur.nl.

Abstract

The increasing demand for environmentally friendly production processes of green chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR-Cas-based tools for genome editing and expression control have enabled fast, easy, and accurate strain development for established production platform organisms, such as Escherichia coli and Saccharomyces cerevisiae. However, the growing interest in alternative production hosts, for which genome editing options are generally limited, requires further developing such engineering tools. In this review, we discuss established and emerging CRISPR-Cas-based tools for genome editing and transcription control of model and non-model prokaryotes, and we analyse the possibilities for further improvement and expansion of these tools for next generation prokaryotic engineering.

KEYWORDS:

CRISPR-Cas; Cas9; archaea; bacteria; genome editing; recombineering

PMID:
26944793
DOI:
10.1016/j.tibtech.2016.02.004
[Indexed for MEDLINE]

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