Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit

Ioannis Mougiakos; Elleke F Bosma; Willem M de Vos; Richard van Kranenburg; John van der Oost

doi:10.1016/j.tibtech.2016.02.004

Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit

Trends Biotechnol. 2016 Jul;34(7):575-587. doi: 10.1016/j.tibtech.2016.02.004. Epub 2016 Mar 2.

Authors

Ioannis Mougiakos¹, Elleke F Bosma¹, Willem M de Vos¹, Richard van Kranenburg², John van der Oost³

Affiliations

¹ Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
² Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands; Corbion, Arkelsedijk 46, 4206 AC Gorinchem, The Netherlands.
³ Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands. Electronic address: john.vanderoost@wur.nl.

PMID: 26944793
DOI: 10.1016/j.tibtech.2016.02.004

Abstract

The increasing demand for environmentally friendly production processes of green chemicals and fuels has stimulated research in microbial metabolic engineering. CRISPR-Cas-based tools for genome editing and expression control have enabled fast, easy, and accurate strain development for established production platform organisms, such as Escherichia coli and Saccharomyces cerevisiae. However, the growing interest in alternative production hosts, for which genome editing options are generally limited, requires further developing such engineering tools. In this review, we discuss established and emerging CRISPR-Cas-based tools for genome editing and transcription control of model and non-model prokaryotes, and we analyse the possibilities for further improvement and expansion of these tools for next generation prokaryotic engineering.

Keywords: CRISPR-Cas; Cas9; archaea; bacteria; genome editing; recombineering.

Publication types

Review

MeSH terms

Bacteria / genetics*
Bacteria / metabolism*
Biotechnology / methods*
CRISPR-Cas Systems
Gene Editing / methods*
Gene Targeting / methods*
Metabolic Engineering / methods*
Recombination, Genetic