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Mod Rheumatol. 2016 Nov;26(6):923-928. doi: 10.3109/14397595.2016.1159385. Epub 2016 Apr 26.

Analysis of IgG4-positive clones in affected organs of IgG4-related disease.

Author information

1
a Division of Rheumatology and.
2
b Division of Cardiology, Department of Internal Medicine , Kanazawa University Graduate School of Medicine , Japan.

Abstract

OBJECTIVE:

We investigated class switch reaction (CSR) in affected organs and evaluated whether the same or genetically related clones exist in IgG4-RD.

METHODS:

We studied three patients with IgG4-RD. Total cellular RNA was extracted from salivary glands and peripheral blood and lung tissue. Activation-induced cytidine deaminase (AID) and immunoglobulin heavy chain third complementarity determining region (IgVH-CDR3) of IgM and IgG4 were detected by reverse transcription polymerase chain reaction (RT-PCR). We analyzed the clonal relationship of infiltrating IgG4-positive cells, as compared with IgM. We determined the existence of common clones among organs and patients.

RESULT:

AID was expressed in salivary glands of all patients and lung tissue in one. Closely related IgVH-CDR3 sequences in infiltrating IgG4-positive cells were detected in salivary glands and lung tissue. Identical IgVH-CDR3 sequence between IgM and IgG4 in salivary glands was detected in one patient, indicating CSR in salivary glands. Identical IgVH-CDR3 sequences of IgG4-positive cells were detected between salivary glands and peripheral blood in two patients. Four identical sequences of IgVH-CDR3 existed between patients. Interestingly, one of the four sequences was detected in all patients.

CONCLUSION:

Our results demonstrate the existence of common antigen(s) shared by patients with IgG4-RD.

KEYWORDS:

CDR3; Common antigen; IgG4-related disease

PMID:
26943245
DOI:
10.3109/14397595.2016.1159385
[Indexed for MEDLINE]

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