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Pathog Dis. 2016 Apr;74(3). pii: ftw013. doi: 10.1093/femspd/ftw013. Epub 2016 Mar 2.

Biofilm formation by multidrug resistant Escherichia coli ST131 is dependent on type 1 fimbriae and assay conditions.

Author information

1
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia Australian Infectious Disease Research Centre, University of Queensland, Brisbane, Queensland 4072, Australia Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.
2
Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia.
3
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia Australian Infectious Disease Research Centre, University of Queensland, Brisbane, Queensland 4072, Australia.
4
Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia School of Biomedical Sciences, Queensland University of Technology, Kelvin Grove, Queensland 4059, Australia makrina.totsika@qut.edu.au.

Abstract

Escherichia coli sequence type 131 (ST131) has emerged as a pandemic lineage of important multidrug resistant pathogens worldwide. Despite many studies examining the epidemiology of ST131, only a few studies to date have investigated the capacity of ST131 strains to form biofilms. Some of these studies have reported contrasting findings, with no specific ST131 biofilm-promoting factors identified. Here, we examined a diverse collection of ST131 isolates for in vitro biofilm formation in different media and assay conditions, including urine from healthy adult women. We found significant differences among strains and assay conditions, which offers an explanation for the contrasting findings reported by previous studies using a single condition. Importantly, we showed that expression of type 1 fimbriae is a critical determinant for biofilm formation by ST131 strains and that inhibition of the FimH adhesin significantly reduces biofilm formation. We also offer direct genetic evidence for the contribution of type 1 fimbriae in biofilm formation by the reference ST131 strain EC958, a representative of the clinically dominant H30-Rx ST131 subgroup. This is the first study of ST131 biofilm formation in biologically relevant conditions and paves the way for the application of FimH inhibitors in treating drug resistant ST131 biofilm infections.

KEYWORDS:

FimH; UTI; flow cell; infection; urine; uropathogenic E. coli

PMID:
26940589
DOI:
10.1093/femspd/ftw013
[Indexed for MEDLINE]

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