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Microb Pathog. 1989 Sep;7(3):165-73.

Identification and genetic analysis of mkaA--a gene of the Salmonella typhimurium virulence plasmid necessary for intracellular growth.

Author information

1
Molecular Biology Unit, National Public Health Institute, Helsinki, Finland.

Abstract

Salmonella typhimurium, like many other Salmonella serovars, harbours a large plasmid required for mouse virulence and growth of bacteria in host cells. The nature of one virulence-abolishing Tn5 insertion (zzx-2556::Tn5) in the plasmid was characterized. A 3.7 kb insert harbouring this region was cloned in pBR325. Plasmid-directed protein synthesis in minicells indicated that the transposon had eliminated the expression of a 70 kDa protein encoded by the virulence plasmid. Nucleotide sequence analysis of the corresponding wild type DNA region showed a 1773 bp open reading frame (mkaA), encoding a protein of 591 amino acid residues and a predicted molecular mass of 60.6 kDa; zzx-2556::Tn5 was situated within mkaA.

PMID:
2693884
DOI:
10.1016/0882-4010(89)90052-1
[Indexed for MEDLINE]

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