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Angew Chem Int Ed Engl. 2016 Apr 4;55(15):4826-30. doi: 10.1002/anie.201511781. Epub 2016 Mar 3.

Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides.

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Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103, Leipzig, Germany.
Institut für Bioanalytische Chemie, Universität Leipzig, Deutscher Platz 5, 04103, Leipzig, Germany.
Leibniz-Institut für Oberflächenmodifizierung (IOM), and Wilhelm-Ostwald-Institut für Physikalische und Theoretische Chemie, Universität Leipzig, Permoserstrasse 15, 04318, Leipzig, Germany.
Deutsches Krebsforschungszentrum, Department Medizinische Physik in der Radiologie, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.
Institut für Biochemie, Universität Leipzig, Brüderstrasse 34, 04103, Leipzig, Germany.


Promotion of cell adhesion on biomaterials is crucial for the long-term success of a titanium implant. Herein a novel concept is highlighted combining very stable and affine titanium surface adhesive properties with specific cell binding moieties in one molecule. A peptide containing L-3,4-dihydroxyphenylalanine was synthesized and affinity to titanium was investigated. Modification with a cyclic RGD peptide and a heparin binding peptide (HBP) was realized by an efficient on-resin combination of Diels-Alder reaction with inverse electron demand and Cu(I) catalyzed azide-alkyne cycloaddition. The peptide was fluorescently labeled by thiol Michael addition. Conjugating the cyclic RGD and HBP in one peptide gave improved spreading, proliferation, viability, and the formation of well-developed actin cytoskeleton and focal contacts of osteoblast-like cells.


DOPA; cell adhesion; click chemistry; peptides; surface chemistry

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