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Molecules. 2016 Mar 1;21(3):123. doi: 10.3390/molecules21030123.

Novel Gold Nanoparticles Reduced by Sargassum glaucescens: Preparation, Characterization and Anticancer Activity.

Author information

1
Innovation Center for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia. z_azhdari@yahoo.com.
2
Darou Pakhsh Pharmaceutical Manufacturing Company, St 61th Daroupakhsh City, Km 18- Karaj Hiway, 1411816616 Tehran, Iran. z_azhdari@yahoo.com.
3
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. heshusr77@gmail.com.
4
College of Veterinary Medicine, University of Sulaimani, Street 11, Zone 207, Sulaimani Nwe, 00964 Sulaimani City, Kurdistan Region, Iraq. heshusr77@gmail.com.
5
Malaysia-Japan International Institute of Technology, Universiti Technology Malaysia, Jalan Sultan Yahya Ahmad Petra, 54100 Kuala Lumpur, Malaysia. kamyarshameli@gmail.com.
6
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. rasedee@gmail.com.
7
Innovation Center for Confectionery Technology (MANIS), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor Darul Ehsan, Malaysia. maaruf71@ukm.edu.my.
8
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. skyeap2005@gmail.com.
9
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. upmsahar@yahoo.com.
10
Iranian Fisheries Research Organization, No. 297, West Fatemi Avenue, P. O. Box 14155-6116, 1411816616 Tehran, Iran. danielajdari@yahoo.com.
11
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. arbarif@biotech.upm.edu.my.

Abstract

The current study investigated the anticancer properties of gold nanoparticles (SG-stabilized AuNPs) synthesized using water extracts of the brown seaweed Sargassum glaucescens (SG). SG-stabilized AuNPs were characterized by ultraviolet-visible spectroscopy, transmission and scanning electron microscopy, and energy dispersive X-ray fluorescence spectrometry. The SG-stabilized AuNPs were stable and small at 3.65 ± 1.69 nm in size. The in vitro anticancer effect of SG-stabilized AuNPs was determined on cervical (HeLa), liver (HepG2), breast (MDA-MB-231) and leukemia (CEM-ss) cell lines using fluorescence microscopy, flow cytometry, caspase activity determination, and MTT assays. After 72 h treatment, SG-stabilized AuNPs was shown to be significant (p < 0.05) cytotoxic to the cancer cells in a dose- and time-dependent manner. The IC50 values of SG-stabilized AuNPs on the HeLa, HepG2, CEM-ss, MDA-MB-231 cell lines were 4.75 ± 1.23, 7.14 ± 1.45, 10.32 ± 1.5, and 11.82 ± 0.9 μg/mL, respectively. On the other hand, SG-stabilized AuNPs showed no cytotoxic effect towards the normal human mammary epithelial cells (MCF-10A). SG-stabilized AuNPs significantly (p < 0.05) arrest HeLa cell cycle at G2/M phase and significantly (p < 0.05) activated caspases-3 and -9 activities. The anticancer effect of SG-stabilized AuNPs is via the intrinsic apoptotic pathway. The study showed that SG-stabilized AuNPs is a good candidate to be developed into a chemotherapeutic compound for the treatment of cancers especially cervical cancer.

KEYWORDS:

Sargassum glaucescens; anticancer properties; cervical cancer; gold nanoparticles; in vitro study

PMID:
26938520
DOI:
10.3390/molecules21030123
[Indexed for MEDLINE]
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