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PLoS One. 2016 Mar 3;11(3):e0149291. doi: 10.1371/journal.pone.0149291. eCollection 2016.

Donor Dependent Variations in Hematopoietic Differentiation among Embryonic and Induced Pluripotent Stem Cell Lines.

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INSERM UMR935, SFR André Lwoff, University Paris Sud, Paul Brousse Hospital, Villejuif, France.
Human Embryonic Stem Cell Core Facility, ESTeam Paris Sud, Villejuif, France.
Department of Pediatrics and Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Science Center, Amarillo, TX, United States of America.
INSERM UMR U1170, Institut Gustave Roussy, Villejuif, France.
AP-HP, Department of Hematology, University Hospitals Paris Sud, Paul Brousse Hospital, Villejuif, France.
Faculty of Medicine, Kremlin-Bicêtre, University, Paris Sud, Paris Saclay, France.
Sanofi, Department of Regenerative Medicine, Global Biotherapeutics, Vitry-sur-Seine, France.
INSERM/UMR S933, University Pierre and Marie Curie-Paris 6, Paris, France.
INSERM UMR 972, SFR André Lwoff, Paul Brousse, Hospital, Villejuif, University Paris Sud, Paris Saclay, France.
National Infrastructure INGESTEM, University Paris Sud, Inserm, Paris, France.


Hematopoiesis generated from human embryonic stem cells (ES) and induced pluripotent stem cells (iPS) are unprecedented resources for cell therapy. We compared hematopoietic differentiation potentials from ES and iPS cell lines originated from various donors and derived them using integrative and non-integrative vectors. Significant differences in differentiation toward hematopoietic lineage were observed among ES and iPS. The ability of engraftment of iPS or ES-derived cells in NOG mice varied among the lines with low levels of chimerism. iPS generated from ES cell-derived mesenchymal stem cells (MSC) reproduce a similar hematopoietic outcome compared to their parental ES cell line. We were not able to identify any specific hematopoietic transcription factors that allow to distinguish between good versus poor hematopoiesis in undifferentiated ES or iPS cell lines. There is a relatively unpredictable variation in hematopoietic differentiation between ES and iPS cell lines that could not be predicted based on phenotype or gene expression of the undifferentiated cells. These results demonstrate the influence of genetic background in variation of hematopoietic potential rather than the reprogramming process.

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