Format

Send to

Choose Destination
J Virol. 2016 Apr 29;90(10):5200-5204. doi: 10.1128/JVI.03174-15. Print 2016 May 15.

The RNA Helicase eIF4A Is Required for Sapovirus Translation.

Author information

1
Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Buk-gu, Gwangju, Republic of Korea.
2
Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom.
3
Department of Life Sciences, Imperial College London, South Kensington, London, United Kingdom.
4
Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom ig299@cam.ac.uk choko@chonnam.ac.kr.
5
Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Buk-gu, Gwangju, Republic of Korea ig299@cam.ac.uk choko@chonnam.ac.kr.

Abstract

The eukaryotic initiation factor 4A (eIF4A) is a DEAD box helicase that unwinds RNA structure in the 5' untranslated region (UTR) of mRNAs. Here, we investigated the role of eIF4A in porcine sapovirus VPg-dependent translation. Using inhibitors and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5' UTR, eIF4A is required to unwind RNA structure in the sapovirus genome to facilitate virus translation.

PMID:
26937032
PMCID:
PMC4859703
DOI:
10.1128/JVI.03174-15
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center