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J Neurol Sci. 1989 Dec;94(1-3):125-36.

Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody.

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Muscular Dystrophy Group Research Laboratory, Newcastle General Hospital, Newcastle upon Tyne, U.K.


The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.

[Indexed for MEDLINE]

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