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Nano Lett. 2016 Apr 13;16(4):2781-5. doi: 10.1021/acs.nanolett.6b00530. Epub 2016 Mar 3.

Light-Triggered Release of Bioactive Molecules from DNA Nanostructures.

Author information

1
Biomedical Engineering Department, Boston University , Boston, Massachusetts 02215, United States.
2
Biology Department, Boston University , Boston, Massachusetts 02215, United States.

Abstract

Recent innovations in DNA nanofabrication allow the creation of intricately shaped nanostructures ideally suited for many biological applications. To advance the use of DNA nanotechnology for the controlled release of bioactive molecules, we report a general strategy that uses light to liberate encapsulated cargoes from DNA nanostructures with high spatiotemporal precision. Through the incorporation of a custom, photolabile cross-linker, we encapsulated cargoes ranging in size from small molecules to full-sized proteins within DNA nanocages and then released such cargoes upon brief exposure to light. This novel molecular uncaging technique offers a general approach for precisely releasing a large variety of bioactive molecules, allowing investigation into their mechanism of action, or finely tuned delivery with high temporal precision for broad biomedical and materials applications.

KEYWORDS:

DNA origami; bioactive cargo; controlled release; nanotechnology; photolabile cross-linker; uncaging

PMID:
26935839
PMCID:
PMC4959465
DOI:
10.1021/acs.nanolett.6b00530
[Indexed for MEDLINE]
Free PMC Article

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