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J Biomater Appl. 2016 May;30(10):1589-600. doi: 10.1177/0885328216635838. Epub 2016 Mar 1.

Ibuprofen-conjugated hyaluronate/polygalacturonic acid hydrogel for the prevention of epidural fibrosis.

Author information

1
Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan.
2
Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
3
Department of Orthopedic Surgery, National Taiwan University Hospital.
4
Division of Neurosurgery, Department of Surgery, Cathay General Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan.
5
Department of Biomedical Engineering, National Yang-Ming University, Taipei, Taiwan Division of Neurosurgery, Department of Surgery, Cathay General Hospital, Taipei, Taiwan School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan Department of Biomedical Engineering, Ming Chuan University, Taipei, Taiwan chen.minghong@gmail.com tyliu5@ym.edu.tw.

Abstract

The formation of fibrous tissue is part of the natural healing response following a laminectomy. Severe scar tissue adhesion, known as epidural fibrosis, is a common cause of failed back surgery syndrome. In this study, by combining the advantages of drug treatment with a physical barrier, an ibuprofen-conjugated crosslinkable polygalacturonic acid and hyaluronic acid hydrogel was developed for epidural fibrosis prevention. Conjugation was confirmed and measured by 1D(1)H NMR spectroscopy.In vitroanalysis showed that the ibuprofen-conjugated polygalacturonic acid-hyaluronic acid hydrogel showed low cytotoxicity. In addition, the conjugated ibuprofen decreased prostaglandin E2production of the lipopolysaccharide-induced RAW264.7 cells. Histological data inin vivostudies indicated that the scar tissue adhesion of laminectomized male adult rats was reduced by the application of our ibuprofen-conjugated polygalacturonic acid-hyaluronic acid hydrogel. Its use also reduced the population of giant cells and collagen deposition of scar tissue without inducing extensive cell recruitment. The results of this study therefore suggest that the local delivery of ibuprofenviaa polygalacturonic acid-hyaluronic acid-based hydrogel reduces the possibility of epidural fibrosis.

KEYWORDS:

Epidural fibrosis; hyaluronic acid; ibuprofen; injectable hydrogel; polygalacturonic acid; prostaglandin E2

PMID:
26935813
DOI:
10.1177/0885328216635838
[Indexed for MEDLINE]

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