Format

Send to

Choose Destination
Sci Rep. 2016 Mar 3;6:22489. doi: 10.1038/srep22489.

Cardiac Light-Sheet Fluorescent Microscopy for Multi-Scale and Rapid Imaging of Architecture and Function.

Author information

1
School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, China.
2
Department of Mechanical &Aerospace Engineering, University of California, Los Angeles (UCLA), Los Angeles, CA, USA.
3
Department of Bioengineering, UCLA, Los Angeles, CA, USA.
4
Department of Molecular, Cellular and Integrative Physiology, UCLA, Los Angeles, CA, USA.
5
Division of Cardiology, Department of Medicine, UCLA, Los Angeles, CA.
6
Department of Electrical Engineering, University of Southern California, Los Angeles, CA, USA.
7
Division of Cardiology, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA, USA.
8
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.

Abstract

Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage and superior axial resolution and contrast. We hereby demonstrate cardiac LSFM (c-LSFM) imaging to assess the functional architecture of zebrafish embryos with a retrospective cardiac synchronization algorithm for four-dimensional reconstruction (3-D space + time). By combining our approach with tissue clearing techniques, we reveal the entire cardiac structures and hypertrabeculation of adult zebrafish hearts in response to doxorubicin treatment. By integrating the resolution enhancement technique with c-LSFM to increase the resolving power under a large field-of-view, we demonstrate the use of low power objective to resolve the entire architecture of large-scale neonatal mouse hearts, revealing the helical orientation of individual myocardial fibers. Therefore, our c-LSFM imaging approach provides multi-scale visualization of architecture and function to drive cardiovascular research with translational implication in congenital heart diseases.

PMID:
26935567
PMCID:
PMC4776137
DOI:
10.1038/srep22489
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center