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Int J Cancer. 2016 Jul 15;139(2):363-72. doi: 10.1002/ijc.30068. Epub 2016 Mar 18.

Exome sequencing of oral squamous cell carcinoma in users of Arabian snuff reveals novel candidates for driver genes.

Author information

1
Department of Preventive Dentistry, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.
2
Department of Oncology and Pharmacogenomics, Beijing Genome Institute (BGI), Shenzhen, Republic of China.
3
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Sana'a University, Yemen.
4
Substance Abuse Research Center (SARC), Jazan University, Jazan, Kingdom of Saudi Arabia.
5
Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA.

Abstract

The study sought to identify genetic aberrations driving oral squamous cell carcinoma (OSCC) development among users of shammah, an Arabian preparation of smokeless tobacco. Twenty archival OSCC samples, 15 of which with a history of shammah exposure, were whole-exome sequenced at an average depth of 127×. Somatic mutations were identified using a novel, matched controls-independent filtration algorithm. CODEX and Exomedepth coupled with a novel, Database of Genomic Variant-based filter were employed to call somatic gene-copy number variations. Significantly mutated genes were identified with Oncodrive FM and the Youn and Simon's method. Candidate driver genes were nominated based on Gene Set Enrichment Analysis. The observed mutational spectrum was similar to that reported by the TCGA project. In addition to confirming known genes of OSCC (TP53, CDKNA2, CASP8, PIK3CA, HRAS, FAT1, TP63, CCND1 and FADD) the analysis identified several candidate novel driver events including mutations of NOTCH3, CSMD3, CRB1, CLTCL1, OSMR and TRPM2, amplification of the proto-oncogenes FOSL1, RELA, TRAF6, MDM2, FRS2 and BAG1, and deletion of the recently described tumor suppressor SMARCC1. Analysis also revealed significantly altered pathways not previously implicated in OSCC including Oncostatin-M signalling pathway, AP-1 and C-MYB transcription networks and endocytosis. There was a trend for higher number of mutations, amplifications and driver events in samples with history of shammah exposure particularly those that tested EBV positive, suggesting an interaction between tobacco exposure and EBV. The work provides further evidence for the genetic heterogeneity of oral cancer and suggests shammah-associated OSCC is characterized by extensive amplification of oncogenes.

KEYWORDS:

carcinoma; exome; genetics; head and neck neoplasms; high-throughput nucleotide sequencing; mouth; shammah; smokeless; squamous cell; tobacco

PMID:
26934577
PMCID:
PMC5821466
DOI:
10.1002/ijc.30068
[Indexed for MEDLINE]
Free PMC Article

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