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Oncotarget. 2016 Mar 8;7(10):10990-1001. doi: 10.18632/oncotarget.7770.

New pathogen-specific immunoPET/MR tracer for molecular imaging of a systemic bacterial infection.

Author information

1
Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, Tübingen, Germany.
2
Institute of Medical Microbiology and Hygiene, Eberhard Karls University, Tübingen, Germany.
3
Institute of Medical Microbiology, Virology and Hygiene, Rostock University Hospital, Rostock, Germany.
4
Institute of Pathology, Eberhard Karls University Tübingen, Tübingen, Germany.
5
Biosciences and ISCA Diagnostics Ltd., University of Exeter, Exeter, United Kingdom.
6
CheMatech, Faculté des Sciences Mirande, Dijon, France.
7
Department of Internal Medicine II, University Hospital Tübingen, Tübingen, Germany.

Abstract

The specific and rapid detection of Enterobacteriaceae, the most frequent cause of gram-negative bacterial infections in humans, remains a major challenge. We developed a non-invasive method to rapidly detect systemic Yersinia enterocolitica infections using immunoPET (antibody-targeted positron emission tomography) with [64Cu]NODAGA-labeled Yersinia-specific polyclonal antibodies targeting the outer membrane protein YadA. In contrast to the tracer [18F]FDG, [64Cu]NODAGA-YadA uptake co-localized in a dose dependent manner with bacterial lesions of Yersinia-infected mice, as detected by magnetic resonance (MR) imaging. This was accompanied by elevated uptake of [64Cu]NODAGA-YadA in infected tissues, in ex vivo biodistribution studies, whereas reduced uptake was observed following blocking with unlabeled anti-YadA antibody. We show, for the first time, a bacteria-specific, antibody-based, in vivo imaging method for the diagnosis of a Gram-negative enterobacterial infection as a proof of concept, which may provide new insights into pathogen-host interactions.

KEYWORDS:

64Cu; Immune response; Immunity; Immunology and Microbiology Section; PET/MR; antibody; bacteria; in vivo imaging

PMID:
26934329
PMCID:
PMC4905453
DOI:
10.18632/oncotarget.7770
[Indexed for MEDLINE]
Free PMC Article

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