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Nature. 2016 Mar 10;531(7593):237-40. doi: 10.1038/nature16974.

MARCKS-like protein is an initiating molecule in axolotl appendage regeneration.

Author information

1
DFG Research Center for Regenerative Therapies (CRTD), Technische Universität Dresden, Fetscherstrasse 105, 01307 Dresden, Germany.
2
Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
3
Karolinska Institute, Department of Cell and Molecular Biology, Centre of Developmental Biology for Regenerative Medicine, SE-171 77 Stockholm, Sweden.

Abstract

Identifying key molecules that launch regeneration has been a long-sought goal. Multiple regenerative animals show an initial wound-associated proliferative response that transits into sustained proliferation if a considerable portion of the body part has been removed. In the axolotl, appendage amputation initiates a round of wound-associated cell cycle induction followed by continued proliferation that is dependent on nerve-derived signals. A wound-associated molecule that triggers the initial proliferative response to launch regeneration has remained obscure. Here, using an expression cloning strategy followed by in vivo gain- and loss-of-function assays, we identified axolotl MARCKS-like protein (MLP) as an extracellularly released factor that induces the initial cell cycle response during axolotl appendage regeneration. The identification of a regeneration-initiating molecule opens the possibility of understanding how to elicit regeneration in other animals.

PMID:
26934225
PMCID:
PMC4795554
DOI:
10.1038/nature16974
[Indexed for MEDLINE]
Free PMC Article

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