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Sci Signal. 2016 Mar 1;9(417):re2. doi: 10.1126/scisignal.aad4403.

Occurrence, structure, and evolution of nitric oxide synthase-like proteins in the plant kingdom.

Author information

1
AgroSup Dijon, UMR 1347 Agroécologie, BP 86510, 21000 Dijon, France. Equipe de Recherche Labellisée CNRS 6300, BP 86510, 21000 Dijon, France.
2
Equipe de Recherche Labellisée CNRS 6300, BP 86510, 21000 Dijon, France. Université Bourgogne Franche-Comté, UMR 1347 Agroécologie, BP 86510, 21000 Dijon, France.
3
Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
4
Instituto de Investigaciones Biológicas, UNMdP-CONICET, CC 1245, 7600 Mar del Plata, Argentina.
5
Botanical Institute, Department of Biological Sciences, Universität zu Köln, Köln D-50674, Germany.
6
BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China.
7
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada.
8
BGI-Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China. Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2E1, Canada.
9
Equipe de Recherche Labellisée CNRS 6300, BP 86510, 21000 Dijon, France. Université Bourgogne Franche-Comté, UMR 1347 Agroécologie, BP 86510, 21000 Dijon, France. wendehen@dijon.inra.fr.

Abstract

Nitric oxide (NO) signaling regulates various physiological processes in both animals and plants. In animals, NO synthesis is mainly catalyzed by NO synthase (NOS) enzymes. Although NOS-like activities that are sensitive to mammalian NOS inhibitors have been detected in plant extracts, few bona fide plant NOS enzymes have been identified. We searched the data set produced by the 1000 Plants (1KP) international consortium for the presence of transcripts encoding NOS-like proteins in over 1000 species of land plants and algae. We also searched for genes encoding NOS-like enzymes in 24 publicly available algal genomes. We identified no typical NOS sequences in 1087 sequenced transcriptomes of land plants. In contrast, we identified NOS-like sequences in 15 of the 265 algal species analyzed. Even if the presence of NOS enzymes assembled from multipolypeptides in plants cannot be conclusively discarded, the emerging data suggest that, instead of generating NO with evolutionarily conserved NOS enzymes, land plants have evolved finely regulated nitrate assimilation and reduction processes to synthesize NO through a mechanism different than that in animals.

PMID:
26933064
DOI:
10.1126/scisignal.aad4403
[Indexed for MEDLINE]

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