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Gene. 1989 Dec 14;84(2):463-6.

Gene expression in a cell-free system on the preparative scale.

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  • 1Insitute of Protein Research, Academy of Sciences of the USSR, Moscow Region.


A cell-free system for preparative gene expression is described. It is composed of DNA-free Escherichia coli extract and added plasmid DNA; coupled transcription-translation proceeds with a continuous flow of the feeding solution containing nucleoside triphosphates and amino acids. The system works at a high constant rate for tens of hours. The yield of synthesised proteins after 20-50 h is hundreds of micrograms from 1 ml of the reaction mixture. Electrophoretic analysis of translation products confirms synthesis of proteins of the expected molecular mass.

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