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Sci Rep. 2016 Mar 2;6:22311. doi: 10.1038/srep22311.

Sequence evidence for common ancestry of eukaryotic endomembrane coatomers.

Author information

Bioinformatics Research Laboratory, Department of Biological Sciences, New Campus, University of Cyprus, PO Box 20537, CY-1678 Nicosia, Cyprus.
Department of Molecular Biology &Genetics, Democritus University of Thrace, GR-68100 Alexandroupolis, Greece.
Donnelly Centre for Cellular &Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
Biological Computation &Process Laboratory (BCPL), Chemical Process Research Institute (CPERI), Centre for Research &Technology (CERTH), PO Box 361, GR-57001 Thessalonica, Greece.


Eukaryotic cells are defined by compartments through which the trafficking of macromolecules is mediated by large complexes, such as the nuclear pore, transport vesicles and intraflagellar transport. The assembly and maintenance of these complexes is facilitated by endomembrane coatomers, long suspected to be divergently related on the basis of structural and more recently phylogenomic analysis. By performing supervised walks in sequence space across coatomer superfamilies, we uncover subtle sequence patterns that have remained elusive to date, ultimately unifying eukaryotic coatomers by divergent evolution. The conserved residues shared by 3,502 endomembrane coatomer components are mapped onto the solenoid superhelix of nucleoporin and COPII protein structures, thus determining the invariant elements of coatomer architecture. This ancient structural motif can be considered as a universal signature connecting eukaryotic coatomers involved in multiple cellular processes across cell physiology and human disease.

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