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BMC Genomics. 2016 Mar 1;17:163. doi: 10.1186/s12864-016-2475-y.

Evidence for contribution of common genetic variants within chromosome 8p21.2-8p21.1 to restricted and repetitive behaviors in autism spectrum disorders.

Author information

1
Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University, 130Dong'an Road, Shanghai, 200032, China. 12110700032@fudan.edu.cn.
2
Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University, 130Dong'an Road, Shanghai, 200032, China. 12210700023@fudan.edu.cn.
3
JohnsHopkins University, Baltimore, MD, USA. backer10@jhu.edu.
4
Unit on Statistical Genomics, Intramural Research Program, National Institute of Mental Health, National Institute of Health, Bethesda, MD, USA. backer10@jhu.edu.
5
Unit on Statistical Genomics, Intramural Research Program, National Institute of Mental Health, National Institute of Health, Bethesda, MD, USA. wei.guo3@nih.gov.
6
Department of Cellular and Genetic Medicine, School of Basic Medical Sciences, Fudan University, 130Dong'an Road, Shanghai, 200032, China. saffen@fudan.edu.cn.
7
Unit on Statistical Genomics, Intramural Research Program, National Institute of Mental Health, National Institute of Health, Bethesda, MD, USA. kay1yao@mail.nih.gov.

Abstract

BACKGROUND:

Restricted and Repetitive Behaviors (RRB), one of the core symptom categories for Autism Spectrum Disorders (ASD), comprises heterogeneous groups of behaviors. Previous research indicates that there are two or more factors (subcategories) within the RRB domain. In an effort to identify common variants associated with RRB, we have carried out a genome-wide association study (GWAS) using the Autism Genetic Resource Exchange (AGRE) dataset (n = 1,335, all ASD probands of European ancestry) for each identified RRB subcategory, while allowing for comparisons of associated single nucleotide polymorphisms (SNPs) with associated SNPs in the same set of probands analyzed using all the RRB subcategories as phenotypes in a multivariate linear mixed model. The top ranked SNPs were then explored in an independent dataset.

RESULTS:

Using principal component analysis of item scores obtained from Autism Diagnostic Interview-Revised (ADI-R), two distinct subcategories within Restricted and Repetitive Behaviors were identified: Repetitive Sensory Motor (RSM) and Insistence on Sameness (IS). Quantitative RSM and IS scores were subsequently used as phenotypes in a GWAS using the AGRE ASD cohort. Although no associated SNPs with genome-wide significance (P < 5.0E-08) were detected when RSM or IS were analyzed independently, three SNPs approached genome-wide significance when RSM and IS were considered together using multivariate association analysis. These included the top IS-associated SNP, rs62503729 (P-value = 6.48E-08), which is located within chromosome 8p21.2-8p21.1, a locus previously linked to schizophrenia. Notably, all of the most significantly associated SNPs are located in close proximity to STMN4 and PTK2B, genes previously shown to function in neuron development. In addition, several of the top-ranked SNPs showed correlations with STMN4 mRNA expression in adult CEU (Caucasian and European descent) human prefrontal cortex. However, the association signals within chromosome 8p21.2-8p21.1 failed to replicate in an independent sample of 2,588 ASD probands; the insufficient sample size and between-study heterogeneity are possible explanations for the non-replication.

CONCLUSIONS:

Our analysis indicates that RRB in ASD can be represented by two distinct subcategories: RSM and IS. Subsequent univariate and multivariate genome-wide association studies of these RRB subcategories enabled the detection of associated SNPs at 8p21.2-8p21.1. Although these results did not replicate in an independent ASD dataset, genomic features of this region and pathway analysis suggest that common variants in 8p21.2-8p21.1 may contribute to RRB, particularly IS. Together, these observations warrant future studies to elucidate the possible contributions of common variants in 8p21.2-8p21.1 to the etiology of RSM and IS in ASD.

PMID:
26931105
PMCID:
PMC4774106
DOI:
10.1186/s12864-016-2475-y
[Indexed for MEDLINE]
Free PMC Article

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