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Sci Rep. 2016 Mar 2;6:22569. doi: 10.1038/srep22569.

Specific LED-based red light photo-stimulation procedures improve overall sperm function and reproductive performance of boar ejaculates.

Author information

1
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), Barcelona, Spain.
2
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Level 3, Women's Centre, John Radcliffe Hospital, Headington, Oxford OX3 9DU, United Kingdom.
3
GenIUL, Rambla de Sant Nebridi 22, E-08222 Terrassa, Spain.
4
Servicios Genéticos Porcinos, S.L., E-08150 Les Masies de Roda, Spain.
5
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain.

Abstract

The present study evaluated the effects of exposing liquid-stored boar semen to different red light LED regimens on sperm quality and reproductive performance. Of all of the tested photo-stimulation procedures, the best pattern consisted of 10 min light, 10 min rest and 10 min of further light (10-10-10 pattern). This pattern induced an intense and transient increase in the majority of motility parameters, without modifying sperm viability and acrosome integrity. While incubating non-photo-stimulated sperm at 37 °C for 90 min decreased all sperm quality parameters, this reduction was prevented when the previously-described light procedure was applied. This effect was concomitant with an increase in the percentage of sperm with high mitochondrial membrane potential. When sperm were subjected to 'in vitro' capacitation, photo-stimulation also increased the percentage of sperm with capacitation-like changes in membrane structure. On the other hand, treating commercial semen doses intended for artificial insemination with the 10-10-10 photo-stimulation pattern significantly increased farrowing rates and the number of both total and live-born piglets for parturition. Therefore, our results indicate that a precise photo-stimulation procedure is able to increase the fertilising ability of boar sperm via a mechanism that could be related to mitochondrial function.

PMID:
26931070
PMCID:
PMC4773850
DOI:
10.1038/srep22569
[Indexed for MEDLINE]
Free PMC Article

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