Format

Send to

Choose Destination
Nat Methods. 2016 Apr;13(4):371-378. doi: 10.1038/nmeth.3795. Epub 2016 Feb 29.

One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.

Author information

1
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
2
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.
3
Biological Services Department, Weizmann Institute of Science, Rehovot, Israel.
4
Institut für Biochemie und Molekularbiologie, ZBMZ Universität, Freiburg, Germany.
5
BIOSS Centre for Biological Signalling Studies, Universität Freiburg, Freiburg, Germany.
6
Cell Morphogenesis and Signal Transduction, Deutsches Krebsforschungszentrum (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.
#
Contributed equally

Abstract

The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). Despite the significance of yeast libraries and the immense variety of available tags and regulatory elements, only a few such libraries exist, as their construction is extremely expensive and laborious. To overcome these limitations, we developed a SWAp-Tag (SWAT) method that enables one parental library to be modified easily and efficiently to give rise to an endless variety of libraries of choice. To showcase the versatility of the SWAT approach, we constructed and investigated a library of ∼1,800 strains carrying SWAT-GFP modules at the amino termini of endomembrane proteins and then used it to create two new libraries (mCherry and seamless GFP). Our work demonstrates how the SWAT method allows fast and effortless creation of yeast libraries, opening the door to new ways of systematically studying cell biology.

PMID:
26928762
PMCID:
PMC4869835
DOI:
10.1038/nmeth.3795
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center